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Abstract:
Scopul proiectului este de a obtine materiale hibride de tip enzima/oxid nanostructurat pentru marirea vitezei procesului de biodegradare, avand ca obiectiv final cresterea ferilitatii solului. Pentru atingerea acestui scop, nanotuburi de SiO2 (SiO2_NT) si de titanat de sodiu (TiNTs) vor fi functionalizate cu proteaze, lipaze si amilaze extracelulare ale microorganismelor halofile/halotolerante izolate din salinele Romaniei, de ex. soluri si lacuri saline. Ecoenzimele vor fi izolate, purificate, caracterizate biochimic si imobilizate pe suport inorganic (SiO2_NT si TiNTs). Eficienta materialelor hibride va fi testata evaluandu-se mai intai activitatea biologica a materialelor native. Abilitatea structurilor hibride de a contribui la accelerarea vitezei de biodegradare in modelele de soluri experimentale va fi de asemenea evaluata. Proiectul urmareste obtinerea de date reproductibile privind marirea activitatii enzimatice obtinuta prin imobilizarea proteazelor, amilazelor si lipazelor extracelulare pe matricile tubulare de SiO2 si TiNTs. Rezultatele obtinute sunt menite sa contribuie la biofertilizarea solurilor saline intensiv exploatate. Propunerea noastra de proiect are in vedere: (a) optimizarea proprietatilor texturale a matricilor inorganice (SiO2_NT si TiNTs) in vederea viitoarei imobilizari enzimatice; (b) modificarea suprafetei tubulare cu specii chimic active in vederea imbunatatirii imobilizarii enzimelor; (c) izolarea tulpinilor halofile in vederea extragerii enzimelor tinta; (d) teste cu privire la aderenta/adsorbtia enzimelor; (e) teste enzimatice avand ca aplicatie biofertilizarea solurilor.
Summary:
The project aim is to obtain hybrid materials in form of enzymes/1D oxide nanostructures for enhancement of biodegradation process rates, leading to increased soil fertility. In order to achieve this scope, SiO2 (SiO2_NT) and sodium titanate nanotubes (TiNTs) will be functionalized with extracellular proteases, lipases and amylases of halophilic/halotolerant microorganisms isolated from Romanian saline environments, i.e. saline soils and lakes. Exoenzymes will be isolated, purified, biochemically characterized and immobilized on inorganic support (SiO2_NT and TiNTs). The efficiency of the hybrid materials will be tested for their biologically activity in native enzymatic form. The ability of the novel hybrid structures to contribute at the accelerating rate of biodegradation in experimental soil models will be also evaluated. The project is looking for obtaining reproducible data regarding the enhancement of enzymatic activity obtained by immobilization of extracellular protease, amylase, and lipase on tubular matrix of SiO2 and TiNTs. These results are meant to be a useful support for the biofertilization of intensively exploited or salted soils. Our project proposal is focusing on: (a) optimization of the textural properties of inorganic matrices (SiO2_NT and TiNTs) with respect to the further enzymatic immobilization; (b) modification of tubular surfaces with chemical active species in order to improve the enzymatic immobilization; (c) isolation of halophilic strains for targeted exoenzymes extraction; (d) tests regarding the adherence/adsorption of enzymes; (e) enzymatic tests with application in soils biofertilization.
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