Proiectul este axat pe urmatoarele obiective principale:
- etapa initiala consta in prepararea matricilor anorganice cu morfologie speciala, si anume nanotuburi de SiO2 (SiO2_NT) si nanotuburi de titanat de sodiu (TiNTs),
- obtinerea microorganismelor halofile/halotolerante cu o capacitatea ridicata de productie a exoenzimelor de interes in proiect.
- optimizarea SiO2_NT si a nanotuburilor de TiNTs
- caracterizarea lor completă fizico-chimica, in scopul de a realiza caracteristici adecvate pentru imobilizarea enzimatica ulterioara
- izolarea si purificarea enzimelor din microorganisme halofile / halotolerante.
- obtinerea de materiale hibride pornind de la enzime imobilizare pe matrici oxidice prin absorbtie directa / aderenta pe oxizi
- dezvoltarea unui atasament optim enzimatic pe nanotuburi de SiO2_NT modificate cu grupe functionale.
- teste comparative ale activitatii enzimatice in ceea ce priveste proteaza, lipaza, amilaza imobilizate pe SiO2_NT si TiNTs, in formă nativa si, de asemenea, in unele modele experimentale de soluri,
- validarea biotehnologiei bazate pe materiale hibride cu activitate enzimatica pentru fertilizarea solului.
The project focuses on the following main objectives:
- the initial stage consists in the preparation of inorganic matrices with special morphology, namely SiO2 nanotubes (SiO2_NT) and sodium titanate nanotubes (TiNTs),
- obtaining halophilic / halotolerant microorganisms with a high production capacity of exoenzymes useful for the project purpose.
- Optimization of SiO2_NT and TiNTs nanotubes
- their complete physico-chemical characterization in order to achieve appropriate characteristics for subsequent enzymatic immobilisation
- isolation and purification of enzymes from halophilic / halotolerant microorganisms.
- obtaining hybrid materials starting from immobilised enzymes on oxides matrices by direct adsorption / adhesion on oxides
- development of an optimal enzimatic bonding on SiO2_NT nanotubes modified with functional groups.
- comparative tests of enzyme activity in terms of protease, lipase, amylase immobilized on SiO2_NT and TiNTs in native form, and also in some experimental soil models,
- validation of biotechnology based on hybrid materials with enzymatic activity for soil fertilization.
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